abstract：:The intrinsic kinetics of continuous yeast cell growth and ethanol production for a self-flocculating fusant yeast strain SPSC01 was investigated by means of mechanically dispersing the flocs and correspondingly established floc size distribution on-line monitoring technique using the focused beam reflectance measurem...

journal_title：Journal of biotechnology

authors： Ge XM,Bai FW

更新日期：2006-07-13 00:00:00

abstract：:The synthesis of methyl alpha-D-glucooligosaccharides, using sucrose as glucosyl donor and methyl alpha-D-glucopyranoside as acceptor, was studied with dextransucrase from Leuconostoc mesenteroides NRRL B-1299. The enzyme was immobilized by entrapment in alginate. By NMR and mass spectrometry we identified three homol...

journal_title：Journal of biotechnology

authors： de Segura AG,Alcalde M,Bernabé M,Ballesteros A,Plou FJ

更新日期：2006-07-13 00:00:00

abstract：:It has been well recognized that many key aspects of cell cycle regulation are encoded into the size distributions of growing budding yeast populations due to the tight coupling between cell growth and cell division present in this organism. Several attempts have been made to model the cell size distribution of growin...

journal_title：Journal of biotechnology

authors： Hatzis C,Porro D

更新日期：2006-07-13 00:00:00

abstract：:Previously, we showed that the enzymes aspartokinase (AK) and dihydrodipicolinate synthase (DDPS), which are involved in L-lysine biosynthesis in the Gram-negative obligate methylotroph Methylophilus methylotrophus AS1, were inhibited by allosteric effectors, including L-lysine. To elucidate further the regulation of ...

journal_title：Journal of biotechnology

authors： Tsujimoto N,Gunji Y,Ogawa-Miyata Y,Shimaoka M,Yasueda H

更新日期：2006-07-13 00:00:00

abstract：:During the last years the great importance of RNA for regulating gene expression in all organisms has become obvious. Consequently, several recent approaches aim to utilize the outstanding chemical properties of RNA to develop artificial RNA regulators for conditional gene expression systems. A combination of rational...

journal_title：Journal of biotechnology

authors： Bauer G,Suess B

更新日期：2006-06-25 00:00:00

abstract：:Because of their ecological functions, natural products have been optimized in evolution for interaction with biological systems and receptors. However, they have not necessarily been optimized for other desirable drug properties and thus can often be improved by structural modification. Using examples from the litera...

journal_title：Journal of biotechnology

authors： Floss HG

更新日期：2006-06-25 00:00:00

abstract：:A new current amplified immunosensor for the determination of carcinoembryonic antigen (CEA) was demonstrated in this work. The electrode architecture was fabricated by positively charged toluidine blue (TB) coated on negatively charged poly-sulfanilic acid (PSAA) modified glassy carbon electrode (GCE) surface through...

journal_title：Journal of biotechnology

authors： Li X,Yuan R,Chai Y,Zhang L,Zhuo Y,Zhang Y

更新日期：2006-05-29 00:00:00

abstract：:Recombinant bacteriophage Qbeta coat protein (CP), which has been proposed as a promising carrier of foreign epitopes via their incorporation either by gene engineering techniques or by chemical coupling, efficiently self-assembles into virus-like particles (VLPs) when expressed in Escherichia coli. Here, we demonstra...

journal_title：Journal of biotechnology

authors： Freivalds J,Dislers A,Ose V,Skrastina D,Cielens I,Pumpens P,Sasnauskas K,Kazaks A

更新日期：2006-05-29 00:00:00

abstract：:We established a novel method to analyze the gene expression of prokaryotes by modifying and optimizing the HiCEP (high coverage gene expression analysis) method, which was originally developed for eukaryotic gene expression profiling. Following the removal of abundant rRNA, the mRNA of prokaryotic cells was enzymatic...

journal_title：Journal of biotechnology

authors： Mitani Y,Suzuki K,Kondo K,Okumura K,Tamura T

更新日期：2006-05-29 00:00:00

abstract：:The thermostable glucose dehydrogenase (GDH) from Burkholderia cepacia sp. SM4 is composed of a catalytic subunit (alpha), an electron transfer subunit (beta), and a small gamma subunit of unknown function. We cloned a 1428-nucleotide gene encoding the beta subunit located immediately downstream of the alpha subunit. ...

journal_title：Journal of biotechnology

authors： Tsuya T,Ferri S,Fujikawa M,Yamaoka H,Sode K

更新日期：2006-05-17 00:00:00

abstract：:Recently, some research in the field of enhanced biological phosphorus removal (EBPR) has been focused on studying systems where the electron donor (substrate) and the electron acceptor (nitrate or oxygen) are present simultaneously. This can occur, for example, in a full scale wastewater treatment plant during heavy ...

journal_title：Journal of biotechnology

authors： Pijuan M,Guisasola A,Baeza JA,Carrera J,Casas C,Lafuente J

更新日期：2006-05-03 00:00:00

abstract：:Cell culture optimization is a labor-intensive process requiring a large number of experiments to be conducted under varying conditions. Here we describe a high-throughput bioreactor system that allows 12 mini stirred-tank bioreactors to be operated simultaneously. All bioreactors are monitored by low-cost minimally i...

journal_title：Journal of biotechnology

authors： Ge X,Hanson M,Shen H,Kostov Y,Brorson KA,Frey DD,Moreira AR,Rao G

更新日期：2006-04-10 00:00:00

abstract：:Human interleukin (IL)-29 is the latest member of the class II cytokine family. However, as a result of lacking efficient method to generate relatively large quantity of IL-29, little is known of its functions in man. In the present study, an Escherichia coli expression system for the rapid expression of the human IL-...

journal_title：Journal of biotechnology

authors： Li M,He S

更新日期：2006-04-10 00:00:00

abstract：:The production of polyhydroxyalkanoates from acetate and propionate by two mixed cultures well adapted to each of these substrates was evaluated. Sludge fed with acetate (A), produced a homopolymer of hydroxybutyrate (HB), whereas sludge fed with propionate (P) produced a copolymer of HB and HV (hydoxyvalerate). Switc...

journal_title：Journal of biotechnology

authors： Lemos PC,Serafim LS,Reis MA

更新日期：2006-03-23 00:00:00

abstract：:Generation and characterization of knockout clones is a widely used approach to evaluate the specific function of a gene product in Dictyostelium discoideum. The mutant clones are generally obtained by double homologous recombination in the target gene. A frequent limitation to obtaining mutants is the low frequency o...

journal_title：Journal of biotechnology

authors： Charette SJ,Cornillon S,Cosson P

更新日期：2006-03-09 00:00:00

abstract：:We developed a detailed mathematical model describing the coupling between the molecular weight distribution dynamics of poly(3-hydroxybutyrate-co-3hydroxyvalerate) (PHBV) copolymer chains with those of hydroxybutyrate (HB) and hydroxyvalerate (HV) monomer formation. Sensitivity analysis of the model revealed that bot...

journal_title：Journal of biotechnology

authors： Iadevaia S,Mantzaris NV

更新日期：2006-03-09 00:00:00

abstract：:In this paper, we report the chromium(VI) reduction by filtrates of Acidithiobacillus and Thiobacillus cultures. Chromium(VI) reduction by filtrates of A. ferrooxidans cultures under acidic conditions was higher than that observed for A. thiooxidans. However, at pH close to 7, chromium(VI) reduction by filtrates of T....

journal_title：Journal of biotechnology

authors： Allegretti P,Furlong J,Donati E

更新日期：2006-03-09 00:00:00

abstract：:Myxobacteria are well established as proficient producers of natural products with numerous biological activities. Although some knowledge has been gained regarding the biosynthesis of secondary metabolites in this class of bacteria, almost nothing is known about the underlying regulatory mechanisms. In order to ident...

journal_title：Journal of biotechnology

authors： Rachid S,Sasse F,Beyer S,Müller R

更新日期：2006-02-24 00:00:00

abstract：:Phycocyanin (PC) prepared from a cyanobacterium Spirulina platensis by the DEAE-DE52 cellulose column chromatography that was developed by gradient elution of 50-250 mM phosphate buffer (pH 7.0) was stabilized by its subunits cross-linked covalently with formaldehyde. The single blue band that the chemically stabilize...

journal_title：Journal of biotechnology

authors： Sun L,Wang S,Qiao Z

更新日期：2006-02-24 00:00:00

abstract：:Glucose oxidase (GOD) was immobilized on cellulose acetate-polymethylmethacrylate (CA-PMMA) membrane. The immobilized GOD showed better performance as compared to the free enzyme in terms of thermal stability retaining 46% of the original activity at 70 degrees C where the original activity corresponded to that obtain...

journal_title：Journal of biotechnology

authors： Rauf S,Ihsan A,Akhtar K,Ghauri MA,Rahman M,Anwar MA,Khalid AM

更新日期：2006-02-10 00:00:00

abstract：:An alkalophilic bacterium was isolated based on the potential of extra-cellular enzymes for bioscouring. The bacterium was identified as a new strain of Bacillus pumilus BK2 producing an extra-cellular endo-pectate lyase PL (EC 4.2.2.2). PL was purified to homogeneity in three steps and has a molecular mass of 37.3+/-...

journal_title：Journal of biotechnology

authors： Klug-Santner BG,Schnitzhofer W,Vrsanská M,Weber J,Agrawal PB,Nierstrasz VA,Guebitz GM

更新日期：2006-02-10 00:00:00

abstract：:Phytopathogenic fungi penetrate plants by breaking down the cuticular barrier with cutinase. Cutinases are extracellular hydrolytic enzymes that degrade cutin, a polyester composed of hydroxy and epoxy fatty acids. Until now, cutinase has been recognized by its ability to release labeled cutin monomers or by a non-spe...

journal_title：Journal of biotechnology

authors： Degani O,Salman H,Gepstein S,Dosoretz CG

更新日期：2006-02-10 00:00:00

abstract：:Insertion of foreign DNA into plant genomes occurs randomly and with low frequency. Hence, a selectable marker is generally required to identify transgenic plants. Until now, all selection systems have been based on the use of non-plant genes, derived from microorganisms and usually conferring antibiotic or herbicide ...

journal_title：Journal of biotechnology

authors： Leyman B,Avonce N,Ramon M,Van Dijck P,Iturriaga G,Thevelein JM

更新日期：2006-02-10 00:00:00

abstract：:The purification of proteins from complex cell culture samples is an essential step in proteomic research. Traditional chromatographic methods often require several steps resulting in time consuming and costly procedures. In contrast, protein purification via membrane adsorbers offers the advantage of fast and gentle ...

journal_title：Journal of biotechnology

authors： Suck K,Walter J,Menzel F,Tappe A,Kasper C,Naumann C,Zeidler R,Scheper T

更新日期：2006-02-10 00:00:00

abstract：:Myxobacteria belonging to the genus Sorangium are known to produce a variety of biologically active secondary metabolites. Chivosazol is a macrocyclic antibiotic active against yeast, filamentous fungi and especially against mammalian cells. The compound specifically destroys the actin skeleton of eucaryotic cells and...

journal_title：Journal of biotechnology

authors： Perlova O,Gerth K,Kaiser O,Hans A,Müller R

更新日期：2006-01-24 00:00:00

abstract：:MutS as a mismatch binding protein is a promising tool for SNP detection. Green fluorescent protein (GFP) is known as an excellent reporter domain. We constructed chimeric proteins consisting of MutS from Thermus thermophilus and GFPuv from Aequorea victoria by cloning the GFPuv gene into the plasmid vectors carrying ...

journal_title：Journal of biotechnology

authors： Stanisławska-Sachadyn A,Sachadyn P,Ihle K,Sydorczuk C,Wiejacha K,Kur J

更新日期：2006-01-24 00:00:00

abstract：:The B subunit of Escherichia coli heat-labile toxin (LTB) is a potent mucosal immunogen and immunoadjuvant for co-administered antigens. In order to produce large scale of LTB for the development of edible vaccine, we used transgenic somatic embryos of Siberian ginseng, which is known as medicinal plant. When transgen...

journal_title：Journal of biotechnology

authors： Kang TJ,Lee WS,Choi EG,Kim JW,Kim BG,Yang MS

更新日期：2006-01-24 00:00:00

abstract：:Spent refinery processing catalyst is listed as a hazardous waste; the toxicity characteristic leaching procedure (TCLP) extracts of the catalyst are found to contain heavy metals at concentrations exceeding the regulated levels. In the present investigation, Aspergillus niger was adapted to single metal ions Ni, Mo o...

journal_title：Journal of biotechnology

authors： Santhiya D,Ting YP

更新日期：2006-01-02 00:00:00

abstract：:Microbial isolates from biofilters and petroleum-polluted bioremediation sites were screened for the presence of enantioselective epoxide hydrolases active towards tert-butyl glycidyl ether, benzyl glycidyl ether, and allyl glycidyl ether. Out of 270 isolated strains, which comprised bacteria, yeasts, and filamentous ...

journal_title：Journal of biotechnology

authors： Kotik M,Brichac J,Kyslík P

更新日期：2005-12-06 00:00:00

abstract：:This study was performed to determine if Akebia and Aristolochia species (Akebia quinata Decaisne and Aristolochia manshuriensis Kom.) could be identified by genetic analysis of Pyrosequencing method, which is used to assess genetic variation. The Pyrosequencing results of Akebia and Aristolochia species showed differ...

journal_title：Journal of biotechnology

authors： Han S,Kim S,Yang CH,Seo J

更新日期：2005-12-06 00:00:00

abstract：:Beta-glucosidase genes of fungal origins were isolated and heterologously expressed in Saccharomyces cerevisiae to enable growth on the disaccharide, cellobiose. To promote secretion of the beta-glucosidases, the genes were fused to the secretion signal of the Trichoderma reesei xyn2 gene and constitutively expressed ...

journal_title：Journal of biotechnology

authors： van Rooyen R,Hahn-Hägerdal B,La Grange DC,van Zyl WH

更新日期：2005-11-21 00:00:00

abstract：:A system for streptavidin detection using biotin conjugated to nano-sized bacterial magnetic particles (BMPs) has been developed. BMPs, isolated from magnetic bacteria, were used as magnetic markers for magnetic force microscopy (MFM) imaging. The magnetic signal was obtained from a single particle using MFM without a...

journal_title：Journal of biotechnology

authors： Amemiya Y,Tanaka T,Yoza B,Matsunaga T

更新日期：2005-11-21 00:00:00

abstract：:Chaperone co-expression and the fusion to different tags were used to modify the aggregation pattern of the putative serine protease CLIPB14 precipitated in Escherichia coli inclusion bodies. A set of common tags used in expression vectors has been selected, as well as two bacterial strains over-expressing the chapero...

journal_title：Journal of biotechnology

authors： Schrödel A,Volz J,de Marco A

更新日期：2005-10-17 00:00:00

abstract：:Production of biopharmaceuticals from mammalian cells requires generation of master, working and post-production cell banks of high quality under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to establishing production processes that are comp...

journal_title：Journal of biotechnology

authors： Fieder J,Wagner N,Grammatikos S,Hoffmann H,Kaufmann H,Otto RT

更新日期：2005-10-17 00:00:00

abstract：:In Salvia miltiorrhiza hairy root cultures, the desired secondary metabolites diterpenoid tanshinones are normally produced at low yields and stored within the roots. To enhance tanshinone production and the secondary product recovery, we employed three means, elicitation with a yeast elicitor (YE), in situ adsorption...

journal_title：Journal of biotechnology

authors： Yan Q,Hu Z,Tan RX,Wu J

更新日期：2005-10-10 00:00:00

abstract：:Two electronic tongues based on different measurement techniques were applied to the discrimination of four molds and one yeast. Chosen microorganisms were different species of Aspergillus and yeast specie Zygosaccharomyces bailii, which are known as food contaminants. The electronic tongue developed in Linköping Univ...

journal_title：Journal of biotechnology

authors： Söderström C,Rudnitskaya A,Legin A,Krantz-Rülcker C

更新日期：2005-09-29 00:00:00

abstract：:The gene encoding histamine dehydrogenase in Rhizobium sp. 4--9 has been cloned and overexpressed in Escherichia coli. The coding region of the gene was 2,079 bp and encoded a protein of 693 amino acids with a calculated molecular mass of 76,732 Da. This histamine dehydrogenase was related to histamine dehydrogenase f...

journal_title：Journal of biotechnology

authors： Bakke M,Sato T,Ichikawa K,Nishimura I

更新日期：2005-09-29 00:00:00

abstract：:Rice straw is a by-product of rice production, and a great bioresource as raw biomass material for manufacturing value-adding protein for animal feedstock, which has been paid more and more attention. In the present work, utilizing rice straw hydrolysate as a substrate for microbial biomass production in 11.5L externa...

journal_title：Journal of biotechnology

authors： Zheng YG,Chen XL,Wang Z

更新日期：2005-09-10 00:00:00

abstract：:Tissue engineering involves ex vivo seeding of anchorage-dependent mammalian cells onto scaffolds, or transplanting cells in vivo. The cell expansion currently requires repeated cell detachment from solid substrata by enzymatic, chemical or mechanical means. The report here presents a high yield three-dimensional cult...

journal_title：Journal of biotechnology

authors： Chia SM,Lin PC,Quek CH,Yin C,Mao HQ,Leong KW,Xu X,Goh CH,Ng ML,Yu H

更新日期：2005-09-10 00:00:00

abstract：:Monitoring cell growth is crucial to the success of an animal cell culture process that can be accomplished by a variety of direct or indirect methodologies. Glucose is a major carbon and energy source for cultured mammalian cells in most cases, but glycolytic metabolism often results in the accumulation of lactate. G...

journal_title：Journal of biotechnology

authors： Tsao YS,Cardoso AG,Condon RG,Voloch M,Lio P,Lagos JC,Kearns BG,Liu Z

更新日期：2005-08-22 00:00:00